Table 1 Sequences of primers used for quantitative real-time PCR
From: Insulin exerts direct, IGF-1 independent actions in growth plate chondrocytes
Gene | DNA sequence (5′–3′) | |
Sox-9 | Forward | GCCAGGTGCTCAAAGGCTA |
Reverse | TCTCGTTCAGAAGTCTCCAGAG | |
Col2α1 | Forward | ACACTGGGACTGTCCTCTG |
Reverse | GTCCAGGGGCACCTTTTTCA | |
Col10α1 | Forward | ATGCTGCCACAAATACCCTTT |
Reverse | GGAATGAAGAACTGTGTCTTGGT | |
IGF-1 | Forward | TGGATGCTCTTCAGTTCGTG |
Reverse | CACTCATCCACAATGCCTGT | |
IGF-2 | Forward | CTTCTCCTCCGATCCTCCTG |
Reverse | TGAGAAGCACCAACATCGAC | |
β-actin | Forward | GTTGTCGACGACGAGCG |
Reverse | GCACAGAGCCTCGCCTT |
