Figure 7

Release of Smac(A54M)-YFP is not impaired by XIAP. (a, b) Representative Cerulean-expressing (a) or C-XIAP-C-expressing (b) HCT Smac^−/− cells releasing Smac(A54M)-YFP in response to 100 ng/ml TRAIL+1 μg/ml CHX are shown. Upon release, Smac(A54M)-YFP fluorescence redistributed from the mitochondria into the cytosol. Onset of release was set to time zero. Release was complete in all cells investigated. Scale bar=10 μm. (c) The duration of Smac(A54M)-YFP release was quantified for all HCT Smac^−/− cells measured. Data are means from n=4 Cerulean-expressing and n=6 C-XIAP-C-expressing cells. Error bars represent S.E.M. (NS, not significant; Student's t-test)