Figure 3 | Cell Death & Disease

Figure 3

From: Cannabidiol causes activated hepatic stellate cell death through a mechanism of endoplasmic reticulum stress-induced apoptosis

Figure 3

Pro-apoptotic effect of CBD is specific to activated HSCs. (a) HSCs from control or ethanol-fed rats, (b) primary mouse in vivo-activated HSCs, (c) LX-2 cells (human HSC line derived from cirrhotic patient), and (d) primary rat hepatocytes were incubated in serum-free media with 5 μM CBD for 0–8 h. Cell lysates were subjected to western blot analysis and probed for PARP, calnexin, and CHOP to detect cell death and ER stress. (e) Acid phosphatase activity was measured in order to determine cell viability following treatment with indicated concentrations of CBD for 8 h in HSCs from control rats, HSCs from ethanol-fed rats, and primary rat hepatocytes. Inset, western blot analysis of α-SMA expression in HSCs from either control or ethanol-fed rats. Western blots and cell survival assays are representative of n=three independent experiments

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