Figure 1
Spermidine-feeding confers paraquat resistance to female Drosophila and improves locomotor activity in female and male flies. (a and b) Survival curve for female (a) and male (b) wild-type flies exposed to 20 mM paraquat and treated with either no spermidine (control), 0.01, 0.1 or 1 mM spermidine (spd.). Results represent the pooled data of five independent replicate experiments. (c and d) Relative mean survival±S.E.M. for female (c) and male (d) wild-type flies exposed to 20 mM paraquat and treated with either no spermidine (control), 0.01, 0.1 or 1 mM spd. Mean survival was normalized to the untreated control. Results represent the pooled data of five independent replicate experiments (**P<0.01, ***P<0.001). (e and f) Survival curve for female (e) and male (f) wild-type flies exposed to 5 mM paraquat and treated with either no spd. (control) or 0.1 mM spd. Results represent the pooled data of three independent replicate experiments. (g) Relative mean survival±S.E.M. for female and male wild-type flies exposed to 5 mM paraquat and treated with either no spd. (control) or 0.1 mM spd. Mean survival was normalized to the untreated control. Results represent the pooled data of three independent replicate experiments (**P<0.01). (h and i) Climbing activity curve for female (h) and male (i) wild-type flies exposed to 5 mM paraquat and treated with or without 0.1 mM spd. Results show the percentage of flies able to climb 8 cm of the vial in 10 s and represent the pooled data of three independent replicate experiments (*P<0.05; **P<0.01; ***P<0.001)
