Figure 4 | Cell Death & Disease

Figure 4

From: Grainyhead-like 2 (GRHL2) inhibits keratinocyte differentiation through epigenetic mechanism

Figure 4

GRHL2 transduction inhibits EDC gene expression in differentiating keratinocytes. (a) Rapidly proliferating NHEK/LXSN and NHEK/GRHL2 were exposed to 1.5 mM Ca++ for 3 days, and qRT-PCR was performed for EDC genes as well as GRHL1 and GRHL3. Bars represent the means of triplicates and S.D. for each gene. Gene expression data in the GRHL2 and LXSN groups (with or without Ca++ treatment) were compared in pairwise manner (P<0.01 for all tested genes). (b) HaCaT cells were exposed to 2 mM Ca++ for up to 10 days and harvested for western blotting after various time points for GRHL2 and IVL. GAPDH (glyceraldehyde 3-phosphate dehydrogenase) was probed as loading control. (c) PMP assay was performed for GRHL3, IVL, and PCNA promoter regions using the HaCaT nuclear extracts collected with or without 72 h exposure to Ca++

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