Figure 4

Inhibition of PI3K/AKT pathway, but not of JAK2, in SOCS1 and SOCS3 clones increases their sensitivity to TNF-α-induced apoptosis. (a) SOCS1 (, n=6) and SOCS3 (, n=6) clones, together with MOCK (, n=6) and SOCS2 (, n=6) clones used as control, were pre-incubated or not with increasing doses (3 and 15 μM final concentration) of the chemical PI3K inhibitor, Ly294002, and then left untreated or stimulated with TNF-α for 48 h. (b) Similarly, MOCK 1.1, SOCS1 1.4, SOCS2 3.1 and SOCS3 6.6 clones were pre-treated or not with increasing doses (from 5 to 30 μM) of PD98059 (ERK1/2 inhibitor) or AG490 (JAK2 inhibitor), and then stimulated with TNF-α. In all experiments, apoptosis was evaluated by measuring Annexin/PI fluorescent staining. Data are expressed as F.I. between TNF-α-treated cells and untreated samples, to which a value of 1 was given. Values of P<0.05 (*) and P<0.01 (**) were considered significant