Figure 6
From: Anti-apoptotic effects of suppressor of cytokine signaling 3 and 1 in psoriasis
Expression of phospho-AKT, phospho-p65, phospho-BAD and BCL-XL is upregulated in activated psoriatic keratinocytes. (a) Immunohistochemistry for p-AKT (Ser473) and p-BAD (Ser136) were performed on frozen sections from biopsies of healthy (n=4) and psoriatic (n=4) skin, including NLS and LS areas of plaques. Phospho-p65 and BCL-XL in vivo staining was performed on paraffin-embedded sections of healthy, NL and LS skin biopsies. Sections were counterstained with Mayer’s H&E. One out of four representative staining of psoriatic skin is shown. (b) Phospho-AKT (Ser473) level was detected in protein lysates of cultured healthy and psoriatic keratinocytes treated with IFN-γ/TNF-α for 12 h. Filter was stripped and re-probed with anti-AKT Ab. One out of six representative healthy and psoriatic keratinocyte strains is shown
