Figure 7

SOCS1 and SOCS3 depletion, as well as the chemical inhibition of PI3K/AKT axis, in activated psoriatic keratinocytes revert their resistance to IFN-γ/TNF-α-induced cell death. (a) Cultured healthy (□, n=6) and psoriatic (▵, n=6) keratinocytes were left untreated or treated with IFN-γ/TNF-α for 48 h. (b) Cultured psoriatic keratinocytes (n=6) were transfected with increasing doses of SOCS1 plus SOCS3 siRNA (▵) or irrelevant siRNA () (20 and 80 nM), then left untreated or treated with IFN-γ/TNF-α for 48 h. (c) Cultured healthy (□, n=6) and psoriatic (▵, n=6) keratinocytes were pre-incubated with increasing doses of Ly294002 (15 and 25 μM) for 2 h and then left untreated or treated with IFN-γ/TNF-α for 48 h. In all experiments, apoptosis was evaluated by measuring Annexin/PI fluorescent staining. Data were expressed as ratio between TNF-α-treated cells and untreated samples. Values of P<0.05 (*) and P<0.01 (**) were considered significant