Figure 6

AMPK-α1-knockdown THP-1 cells exhibit a marked reduction in NF-κB-dependent genes in response to TNF-α stimulation. (A) The wt THP-1 and AMPK-α1^KD THP-1 cells were treated with or without TNF-α (100 ng/ml) for 12 h. Total RNAs were isolated from each sample and microarray analysis was performed as described in Materials and Methods. The 96 NF-κB-dependent upregulated and downregulated genes were sorted and represented: a, wt THP-1 cells treated with TNF-α versus wt THP-1 cells; b, AMPK-α1^KD THP-1 cells treated with TNF-α versus AMPK-α1^KD THP-1 cells; c, AMPK-α1^KD THP-1 cells versus wt THP-1 cells; and d, AMPK-α1^KD THP-1 cells treated with TNF-α versus wt THP-1 cells treated with TNF-α. (B) The wt THP-1 and AMPK-α1^KD THP-1 cells were treated with or without TNF-α (100 ng/ml) for 12 h. Total RNAs were isolated from each sample and quantitative RT-PCR analysis was performed with specific primers targeted to IL-8, TNF-α, IL-1β, CD44, and CCL5 genes. Error bars represent mean±S.D. of triplicate samples