Figure 7
BDNF prevents NMDA-induced phosphorylation of Akt in GFP-p75 STHdh111/111Q mutant cells. Wild-type (ST7/7Q) and mutant (ST111/111Q) huntingtin striatal cells transfected with GFP or GFP-p75 were treated with BDNF (50 ng/ml, 30 min), NMDA (500 μ M, 30 min) or BDNF before addition of NMDA (NMDA+BDNF) for the indicated time periods. Cells were lysed and immunoblots performed to detect Ser-473-Akt (p-Akt) and Akt (a) or phosphoERK1/2 (p-ERK1/2) and ERK1/2 (b). α-tubulin was used as a loading control. The blots are representative of four independent experiments. The histograms represent the relative p-Akt/Akt and p-ERK/ERK ratios considering 100% the ratio obtained in control conditions (vehicle). Values are given as mean±S.E.M. Data was analyzed by Student’s t-test. +P<0.05, ++P<0.01, +++P<0.001 versus vehicle-treated GFP cells, *P<0.05 and ***P<0.001 versus vehicle-treated GFP-p75 cells, $P<0.05, $$$P<0.001 NMDA+BDNF-treated GFP mutant cells versus NMDA+BDNF-treated GFP-p75 mutant cells
