Figure 1

Schematic illustrations. (a) The upper scheme demonstrates how NPCs differentiate into glutamatergic neurons in our culture system. The lower panels are representative images of neurons 7 days after plating in our culture system. Over 97% neurons are positive in VGLUT1 (a glutamatergic neuronal marker), based on immunofluorescence analysis with the neuronal marker Tuj1 (red) and VGLUT1 (green). Nuclei were counterstained with DAPI (blue). Scale bar: 200 μm. (b) Scheme shows the time schedule of ICC and WB analyses after treatment of NPCs with one of the four reagents (TSA, NaB, VPA or VPM) or their vehicle controls at day 0. (c) Schema shows the time schedule of ICC and WB analyses after treating glutamatergic neurons with one of the four reagents or their vehicle control at day 3