Figure 6 | Cell Death & Disease

Figure 6

From: BRCC2 inhibits breast cancer cell growth and metastasis in vitro and in vivo via downregulating AKT pathway

Figure 6

The effects of BRCC2 on the Akt pathway. (a) The expression of Akt and the phosphorylation of Akt at Ser 473 and Thr 308 in BRCC2-transfected cells were examined using western blotting. β-actin was used as a loading control. The expression of each protein was quantified as the densitometry value analyzed by SPSS 18.0 software. (b) The summary graphs show the migration ability of BRCC2 knockdown T47D and its control cells after the cells had been pretreated with the Akt inhibitor LY294002 (100 nM). (c) The transfection efficiency of Akt siRNA 24 h after transfection was measured using western blot analyses. β-Actin was used as a loading control. The expression of each protein was quantified as the densitometry value analyzed by SPSS 18.0 software. (d) The summary graphs show the migration ability of BRCC2 knockdown T47D and its control cells after the cells had been transfected with Akt siRNA and its negative control. The data represent the mean number of cells per field and are presented as the means±S.D. **P<0.01, ***P<0.001

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