Figure 1 | Cell Death & Disease

Figure 1

From: miR-125b transcriptionally increased by Nrf2 inhibits AhR repressor, which protects kidney from cisplatin-induced injury

Figure 1

Increase in miR-125b levels in the kidney by Nrf2. (a) qRT-PCR assays of kidney miRNA levels in mice treated with oltipraz. Mice were gavaged with 30 mg oltipraz/kg/day for 6 days and were killed 1 h after the last dose. miRNAs increased by oltipraz in the lung miRNA database (GSE19540) were subjected to analyses in the kidney. Data represent the mean±S.E.M. (significantly different as compared with vehicle treatment, **P<0.01, NS, not significant; n=4 or 5 per group). (b) Increase of miR-125b by SFN treatment. miR-125b levels were assessed in NRK52E cells treated with 20 μM SFN for 12 h. (c) Increase of miR-125b by Nrf2 overexpression. miR-125b levels were assessed in HEK293A cells transfected with pcDNA3.1 or a construct encoding for Nrf2 for 48 h. (d) Correlation between miR-125b and Nrf2 mRNA levels. miR-125b and Nrf2 transcript levels were compared using human kidney miRNA and cDNA arrays database (GSE16441). For b and c, data represent the mean±S.E.M. of three independent experiments (significantly different as compared with experimental control, **P<0.01)

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