Figure 3 | Cell Death & Disease

Figure 3

From: Differential regulation of the apoptotic machinery during megakaryocyte differentiation and platelet production by inhibitor of apoptosis protein Livin

Figure 3

Functional PLPs are released to the culture medium from differentiating cells. LAMA-84 cells (0.5 × 106) were induced to differentiate to MKs by PMA (5 ng/ml). (a) On day 4, particles from cultured LAMA-84 cells were collected from media and stained with May–Grunwald/Giemsa. Both mature platelets and proplatelets are seen (original magnification, × 20). (b) Normal platelets from peripheral blood were used to establish the forward/side scatter (FSC/SSC) gates using flow cytometric. (c) Subsequent gating of CD41+ normal platelets. (d) Platelets of untreated LAMA84 cells were gated according their physical characteristic FSC/SSC set by control platelets and (e) Subsequent CD41 gating of PLPs from untreated LAMA-84 culture supernatants. (f) PLPs of PMA-treated LAMA-84 cells were gated by FSC/SSC for normal platelets and (g) subsequently by CD41 staining of PLPs from PMA-treated LAMA-84 culture supernatants. (h) Aggregation of normal platelets (top) and LAMA-84-culture derived PLPs (bottom) in response to epinephrine (Epi), collagen (Col), ADP and ristocetin

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