Figure 5 | Cell Death & Disease

Figure 5

From: Differential regulation of the apoptotic machinery during megakaryocyte differentiation and platelet production by inhibitor of apoptosis protein Livin

Figure 5

Cord blood CD34+cells overexpressing Livin show increased differentiation toward the megakaryocytic lineage and higher ploidy. Cord blood CD34+ cells were cultured with TPO and SCF to drive MK differentiation. After one day, the cells were infected with control, WT Livin or RING mutant-expressing retroviruses. (a) At day 14, May–Grunwald/Giemsa staining of cytospin preparations showing polyploid MKs (n=6) (original magnification, × 60). (b) Dot plots of days 0 and 14 differentiated CD34+ cells stained for MK marker (CD41+, n=6). (c) CD41+ cell production was increased in CD34+ cells infected with Livin as compared with CD34+ cells incubated with TPO and SCF alone (n=6). (d) At day 14, the nuclei of MKs generated from CD34+ cells were counted in cytospins of cells stained with May–Grunwald/Giemsa to assess ploidy (n=6). (e) Western blot analysis of Livin protein expression in undifferentiated CD34+ cells (naive) and cells differentiated for 14 days with TPO and SCF and with infected empty vector or plasmid containing Livin. Note the appearance of cleaved proapoptotic tLivin after 14 days of differentiation

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