Figure 7 | Cell Death & Disease

Figure 7

From: Differential regulation of the apoptotic machinery during megakaryocyte differentiation and platelet production by inhibitor of apoptosis protein Livin

Figure 7

The effect of the knockdown of Livin on platelets production and function. Downregulation of Livin expression was performed in both LAMA-84 cells (a–c) and cord blood CD34+ cells (d and e) with the use of pSUPER-Livin-2 or control vector (pSUPER-Luc). (a) Western blot analysis showing the downregulation of both α- and β-Livin expression in LAMA-84 cells. (b) Knockdown of Livin in LAMA-84 cells reduced the number of PLPs (PLPs count/LAMA-84 cells count) (n=5). (c) Aggregation induced by various agonists (ADP (n=7), arachidonic acid (AA) (n=7), epinephrine (Epi) (n=4) and collagen (Col) (n=4)) is reduced in Livin-knockdown cells. (d) At day 14, the nuclei of MKs generated from CD34+ cells were counted in cytospins of cells stained with May–Grunwald/Giemsa (n=3). (e) Platelets derived from CD34+ cells differentiated toward MKs with and without the downregulation of Livin expression were incubated with ADP or AA and were stained with anti-CD62p to detected platelet activation (n=3). Iso, isotype control

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