Figure 1
From: Identification of the NF-κB inhibitor A20 as a key regulator for human adipogenesis
TNFα inhibits adipocytes differentiation from MSCs. (a) Representative staining of lipid droplets by Nile Red. MSCs were induced to differentiate in the absence (control media) or presence of adipogenic medium during 21 days. (b) Side-scatter fluorescence increase correlates with specific fluorescence Nile Red staining (region 1 and 2). (c) MSCs cultured in the presence of increasing concentrations of TNFα. Percentages of mature adipocytes were determined by flow cytometry. Results show the mean±S.D. of triplicate samples. **P<0.01, ***P<0.001. (d) Representative staining of side-scatter fluorescence in the presence/absence of TNFα. Lipid droplet morphology is also shown (400 × magnification). (e) Analysis of apoptosis in TNFα-treated cells induced to differentiate into adipocytes. The percentage of apoptotic cells (Annexin V positive) was measured at 14 and 21-days post-induction by flow cytometry. Results show the means±S.D. of triplicate samples
