Figure 1 | Cell Death & Disease

Figure 1

From: Functional cooperation of miR-125a, miR-125b, and miR-205 in entinostat-induced downregulation of erbB2/erbB3 and apoptosis in breast cancer cells

Figure 1

Treatment with entinostat does not affect mRNA levels of both erbB2 and erbB3 in breast cancer cells. MDA-MB-453 (MDA-453) and BT474 cells untreated or treated with entinostat (ent) at indicated concentrations for 24 h were subjected to total RNA extraction. (a) First-strand cDNA was synthesized using a reverse transcription kit from Applied Biosystems. The partial coding sequence of erbB2, erbB3, or β-actin was amplified with specific primers. The PCR products were separated on a 2% agarose gel containing ethidium bromide and visualized under a UV light. (b) The mRNA levels of erbB2 and erbB3 were measured by qRT-PCR. Bars, S.D. The data are representative of three independent experiments

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