Figure 6

Inactivation of two, but not one miRNA significantly attenuates entinostat-induced apoptosis in erbB2-overexpressing breast cancer cells. MDA-MB-453 and BT474 cells were similarly transfected and then treated with entinostat as described in Figure 4. All cells were collected and subjected to a specific apoptotic ELISA (a) and western blot analyses with specific antibody directed against PARP (F-PARP, full length PARP; C-PARP, cleaved PARP) or β-actin (b). MDA-MB-453 or BT474 cells were simultaneously transfected with two miRNA inhibitors and then treated with entinostat, same as described in Figure 5. All cells were collected and subjected to a specific apoptotic ELISA (c) and western blot analyses with specific antibody directed against PARP or β-actin (d). Bars, S.D. The data are representative of three independent experiments. *P<0.001 versus entinostat treatment with control transfection