Figure 2
Histological analysis of PRSS1 transgenic animals. (a) Representative images of H&E-stained tissue sections of pancreatic tissue isolated from 6-month-old heterozygous animals from each transgenic strain, as indicated, showing examples of vacuolisation that was not present in matchedWT control. (b) Immunohistochemical analysis of the animals analysed in (a) using a PRSS1-specific antibody (AF3848) showing co-localisation between vacuolisation and transgene expression and absence of staining in WT control. (c) Representative images of H&E-stained pancreatic tissue sections isolated from 16-month-old heterozygous animals from each transgenic strain showing infiltration of the pancreas by inflammatory cells associated with the loss of adjacent acinar cells that was not detected in WT control. (d) Immunohistochemical analysis of the same animals analysed in (c) showing that the inflammatory infiltrate coincides with areas of high transgene expression. (e) Representative images of H&E-stained pancreatic tissue sections isolated from 8-month-old homozygous animals from each transgenic strain showing adipose atrophy that was not detected in WT control. (f) Sirius red staining of pancreatic tissue isolated from 12-month-old heterozygous animals from each transgenic strain, showing collagen deposits (red) in the fibrotic reaction resulting from destruction of acinar tissue that was not detected in WT control. Original magnification × 200
