Figure 3
Immunohistochemical analysis of inflammatory lesions observed in pancreatic tissues from PRSS1 transgenic mice. Immunohistochemical analysis of inflammatory infiltrates in 8.5-month-old heterozygous animals from each transgenic strain and age-matched WT control, as indicated, using cell-type-specific marker antibodies for: (a) T cells (CD3), (b) B cells (CD20), (c) macrophages (F4/80) and (d) activated pancreatic stellate cells (PSCs), identified by staining for alpha-smooth muscle actin (α-SMA). Insets represent magnified areas of the photomicrographs (original magnification × 200). (e) WB analysis of pancreatic tissue isolated from littermates derived from an intercross of PRSS1 heterozygous animals using antibodies specific for α-SMA, HA and β actin as indicated. Please note that the same blot was probed for SPINK3, shown in Supplementary Figure 3. P=animal with pancreatitis observed by histological analysis; N, histologically normal animal; wt, wild-type animal of the same genetic background; M, molecular weight marker (kDa)
