Figure 7

Celastrol causes proteotoxicity via a thiol-sensitive mechanism in human glioblastoma. Poly-ubiquitinated protein aggregates along with the autophagy substrate and stress sensor, p62, accumulate as a result of defective protein degradation quality-control mechanisms, namely autophagy and proteasomal degradation. The disruption in proteostasis is further substantiated by the activation of cytoprotective heat-shock responses (HSP72/90)