Figure 3 | Cell Death & Disease

Figure 3

From: A novel androstenedione derivative induces ROS-mediated autophagy and attenuates drug resistance in osteosarcoma by inhibiting macrophage migration inhibitory factor (MIF)

Figure 3

DSTD induces autophagy in U2OS and MG-63 osteosarcoma cells. (a) DSTD induced dose-dependent autophagy. Cells were treated with the indicated concentration of DSTD for 24 h. MIF, Bcl-2, Bax, p-ERK, HMGB1 and LC3 were analyzed by western blot. (b) DSTD induced ROS-mediated autophagy in time-dependent manners. Cells were treated with 100 μM DSTD for indicated time. To determine the effect of NAC on autophagy, after treated with 100 μM DSTD for 24 h, cells continued to be treated with 100 μM DSTD combined with 15 mM NAC (pH 7.4) for 24 h. The cytoplasmic and nucleic fractions were separated. MIF, Bcl-2, Bax, HMGB1, p-ERK, PARP and LC3 were analyzed by western blot. (c) Viability of U2OS and MG-63 cells treated with 100 μM DSTD in the presence or absence of NAC for the indicated time. (d) Cytotoxicity induced by 100 μM DSTD in the presence or absence of NAC for the indicated time. Values are means of at least three independent experiments. *P<0.05 versus control group; #P<0.05 versus group treated with DSTD for 48 h; **P<0.01 versus control group

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