Figure 4 | Cell Death & Disease

Figure 4

From: A novel androstenedione derivative induces ROS-mediated autophagy and attenuates drug resistance in osteosarcoma by inhibiting macrophage migration inhibitory factor (MIF)

Figure 4

DSTD induces ROS generation, LC3 puncta formation and apoptosis in U2OS and MG-63 osteosarcoma cells. (a) Effect of DSTD on ROS generation and LC3 puncta formation. Cells were treated with 100 μM DSTD for 24 h in the presence or absence of 15 mM NAC. After treatment, cells were stained by DCF-DA or LC3 antibody to determine ROS generation and LC3 puncta formation, respectively. (b) Effect of DSTD on cell apoptosis. After treatment, apoptosis was determined by flow cytometry

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