Figure 2
From: Non-apoptotic functions of caspase-7 during osteogenesis
Localisation of activated caspase-7 and osteocalcin in long bones of front limb (ulna, radius) at embryonic and postnatal stages. Morphology of bones of front limb visualised by haematoxylin-eosin and Alcian blue staining at ED14.5 (a), ED15.5 (b), ED17.5 (c), and P6 (d). Localisation of activated (cleaved) caspase-7 in areas of ossification at ED14.5 (e and f), ED15.5 (g and h), ED17.5 (i and j), and P6 (k and l). Expression of osteocalcin in areas of ossification at ED14.5 (m and n), ED15.5 (o and p), ED17.5 (q and r), and P6 (s and t). Arrows point to the positive cells (brown); C7 (caspase-7), hc (hypertrophic chondrocytes), OS (osteocalcin), pb (primary bone), pc (proliferating chondrocytes), and pm (perichondrium). Scale bar=100 μm
