Figure 4

Promoter-specific DNA methylation. (a) Igf2-DMR2, (b) H19 ICR, (c) Pparα, (d) Nr3c1, (e) Pgc1α and (f) Hnf4α P1 were analyzed in the brain, liver and kidney. Data are shown for both embryonic day 13.5 (E13.5) and post-natal day 4 (PN4). White bars show promoter-specific DNA methylation within the control group, gray bars from the NK-depleted group and the black bars from the brain, liver and kidney tissues derived from the DC expanded group. Data represent mean±S.D. derived from 10 to 15 embryos/pups per group. Significant differences are noted as *P<0.05 and **P<0.01 according to ANOVA followed by Tukey post-hoc test