Figure 1
From: Numb-dependent integration of pre-TCR and p53 function in T-cell precursor development
Numb subcellular distribution is dependent on pre-TCR. (a) Confocal images of wild-type (WT) fetal thymus E14d sections stained with antibodies against pTα (red) and Numb (green), and treated with 4',6-diamidino-2-phenylindole (DAPI), which stains the nucleus (blue). Asterisks indicate the pTα-positive cells and arrows indicate the pTα− cells. (Lower panels) Higher magnification of a single DN3 thymocyte (left) and DN2 thymocyte (right). (b and d) Cytosolic and nuclear fractions in (b, upper panel) SCB29 and SCIET27 cells or (d) thymocytes derived from pTα−/− mice were revealed in western blot with anti-numb antibody. Anti-tubulin and anti-laminB antibodies were used as the cytosol and nuclear markers, respectively. C, cytosolic fraction; N, nuclear fraction. (c and e) Confocal images of (c, upper panel) SCB29 and SCIET27 cells or (e) thymocytes derived from pTα−/− mice stained with anti-numb (green or red), and treated with DAPI (blue). (b and c, lower panels) Relative Numb quantification of nuclear and cytosolic Numb: NS, nonsignificant; P-value >0.05. **P-value <0.02. All data are representative of three independent experiments
