Figure 4 | Cell Death & Disease

Figure 4

From: CTGF increases vascular endothelial growth factor-dependent angiogenesis in human synovial fibroblasts by increasing miR-210 expression

Figure 4

Involvement of NF-κB and ELK1 in CTGF promotes miR-210 and VEGF expression as well as angiogenesis. (a) OASFs were transfected with miR-210 luciferase plasmids before incubation with CTGF for 24 h. Luciferase activity was then assayed. (b) OASFs were pretreated with PDTC (10 μM) and TPCK (1 μM) for 30 min or transfected with ELK1 and p65 siRNA for 24 h followed by CTGF stimulation, MiR-210 expression examined by qPCR. (c) OASFs were transfected with ELK1 and p65 siRNA for 24 h followed by stimulation with CTGF. VEGF expression was examined by ELISA. (d and e) Medium was collected as CM and then applied to EPCs for 24 h. Cell capillary-like structure formation and migration in EPCs were examined by tube formation and Transwell assay. (f and g) OASFs were pretreated with Ly294002, Wortmannin, AKTi, U0126, or PD98059 and stimulated with CTGF for 120 min, with p65 and ELK1 phosphorylation in nucleus or p65 and ELK1 binding to miR210 promoter determined by western blot and chromatin immunoprecipitation assay. Results are expressed as the mean±S.E.M. *P<0.05 compared with control; #P<0.05 compared with CTGF-treated group

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