Figure 6 | Cell Death & Disease

Figure 6

From: CTGF increases vascular endothelial growth factor-dependent angiogenesis in human synovial fibroblasts by increasing miR-210 expression

Figure 6

Knockdown of CTGF impairs angiogenesis in vitro and in vivo. (a) OASFs were transfected with control-shRNA or CTGF-shRNA, protein expression of CTGF and VEGF examined by western blot and ELISA. (b and c) Medium collected as CM was applied to EPCs for 24 h. Cell capillary-like structure formation and migration in EPCs were examined by tube formation and Transwell assay. (d) Chick embryos were incubated with PBS, OASFs/control-shRNA CM, or OASFs/CTGF-shRNA CM for 4 days, resected, fixed, and photographed by stereomicroscope. (e) Mice were injected subcutaneously with Matrigel mixed with PBS, OASFs/control-shRNA CM, or OASFs/CTGF-shRNA CM for 7 days. Plugs were excised from mice and photographed, stained with CD31, and hemoglobin content quantified. Results are expressed as mean±S.E.M. *P<0.05 compared with control; #P<0.05 compared with CTGF-treated group

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