Figure 1 | Cell Death & Disease

Figure 1

From: Role of extracellular calcium and mitochondrial oxygen species in psychosine-induced oligodendrocyte cell death

Figure 1

PSY induces dose-dependent cell death in MO3.13 cell line. (a) Representative dot-plots of MO3.13 cells stained with Annexin V/propidium iodide and analyzed by flow cytometry: R8, R9, R7 and R3 quadrants define the healthy, apoptotic, secondary necrotic and necrotic populations, respectively. (b) PSY dose–response quantification of cell viability (n=7, one-way ANOVA (Dunnett’s post-hoc test ) PSY 1–10 μM versus Untreated). (c) Representative nuclear morphologies (imaged by Hoechst staining and fluorescence microscopy) for Untreated (top panel) and PSY-10-μM-treated (bottom panel) cells. The bottom panel inset shows a zoomed image of the nucleus indicated by the white arrow. The presence of condensed/fragmented nuclei is a typical feature of apoptosis induction

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