Figure 5
EDTA treatment extends cell survival and reduces mitochondrial ROS production. (a) Representative dot-plots of MO3.13 cells treated with PSY 10 μM or with PSY 10 μM in presence of EDTA 1 mM, stained with Annexin V/propidium iodide and analyzed by flow cytometry: R8, R9, R7 and R3 quadrants define the healthy, apoptotic, secondary necrotic and necrotic populations, respectively. (b) Cell viability quantification of the flow cytometry dot-plots for cells treated with PSY 3–10 μM, with (+) of without (−) EDTA 1 mM administration (n=7, t-test EDTA (−) versus EDTA (+) for same PSY concentrations). (c) Representative dot-plots of MO3.13 cells treated with PSY 10 μM or with PSY 10 μM in presence of EDTA 1 mM, stained with Mitotracker Red CM-H2XRos and analyzed by flow cytometry. The side-scattered light signal (SSC) is also reported. The R6 region was set by the dot-plot obtained for the control population in (no PSY, 10% FBS). (d) Mitochondrial ROS production quantification of the flow-cytometry dot-plots for cells treated with PSY 3–10 μM, with (+) of without (−) EDTA 1 mM administration (n=4, t-test EDTA (−) versus EDTA (+) for same PSY concentrations)
