Figure 6
Pharmacological reduction of calcium influx and ROS production by EDTA and N-acetyl-cysteine has no synergistic effect on cell survival. (a) Representative dot-plots of MO3.13 cells treated with PSY 10 μM alone, or in combination with EDTA 1 mM, NAC 5 mM and EDTA 1 mM+NAC 5 mM, stained with Annexin V/propidium iodide and analyzed by flow cytometry: R8, R9, R7 and R3 quadrants define the healthy, apoptotic, secondary necrotic and necrotic populations, respectively. (b) Cell viability quantification of the flow cytometry dot-plots for cells treated with PSY 3–10 μM, with (+) of without (−) EDTA 1 mM and NAC 5 mM administration. (n=5, one-way ANOVA, (Tukey post-hot test): EDTA(+)/NAC(−) versus EDTA(−)/NAC(+) versus EDTA(+)/NAC(+) for PSY 3 μM and PSY 10 μM; one-way ANOVA, (Dunns post-hoc test) EDTA(+)/NAC(−) versus EDTA(−)/NAC(+) versus EDTA(+)/NAC(+) for PSY 5 μM)
