Figure 3
From: HMGB1 in the pathogenesis of ultraviolet-induced ocular surface inflammation
UV-induced leukocyte infiltration was inhibited in mouse conjunctiva by HMGB1 A box and sRAGE treatments. (a and b) The conjunctiva of BALB/c mice were exposed to UV (311 nm) at a single dose of 100 mJ/cm2 using a UV lamp 24 h after UV treatment. The whole eye was then enucleated for H&E staining, and the number of infiltrated cells was counted. *P<0.05 (n=10). (b) A representative conjunctival tissue was immunofluorescently stained against Gr-1 (green, upper) or F4/80 (green, lower) for infiltrated leukocytes. DAPI was used to stain the nuclei. (c) Immunohistochemical staining of UV-exposed conjunctival tissue using an anti-HMGB1 antibody. (d) An immunofluorescence assay was performed to observe UV-induced nucleo-cytoplasmic translocation of endogenous HMGB1. (e) BALB/c mice were pretreated with sRAGEs (100 μg per mouse, 15 min before UV radiation) and HMGB1 A box protein (150 μg per injection, 1 h before and after UV radiation), and then treated with a single dose of 100 mJ/cm2 UVB. At 24 h after UV treatment, the whole eyes were enucleated for H&E staining and the infiltrated leukocyte cells were counted. Values shown are the mean±S.E. *P<0.05 (n=10)
