Figure 4
From: HMGB1 in the pathogenesis of ultraviolet-induced ocular surface inflammation
Antioxidant treatment reduced UV-induced infiltration of inflammatory cells to the conjunctiva. BALB/c mice were intraperitoneally treated with NAC (125 mM, n=5) and Mito-Tempo ( Enzo Life Sciences, 135 μM, n=5) and then exposed to 10 mJ/cm2 of UVB. The eyes were enucleated on 24 h after UV treatment. (a) Histologic analysis was performed with H&E staining. The number of leukocyte cells was counted and the mean±S.E. was obtained. *P<0.05. (b) Immunohistochemical staining of the eye tissue using an anti-HMGB1 antibody. (c) Immunofluorescence confocal microscopy was performed with monoclonal antibody of HMGB1 (red) and DAPI (blue). Surface of the cornea epithelium (dotted line)
