Figure 6
From: Survivin, a novel target of the Hedgehog/GLI signaling pathway in human tumor cells
Immunohistochemical and immunofluorescence staining of GLI2 and survivin in human tumors. (a) Double immunofluorescence with anti-GLI2 (rabbit) and anti-survivin (mouse) antibodies demonstrating colocalization of both proteins in the areas of high staining activity of both. The staining has been performed on the frozen tissue (ovarian tumor) after acetone fixation. High correlation has been found using the FIJI software (Spearman's rank correlation value: 0.882). Bar represents 20 μm. (b) Examples of two areas of lung tumors showing the highest score (4) of staining for survivin and GLI2. Parallel sections were stained. The same stained structures are clearly recognized in both lower and higher magnifications. Bars, 100 and 50 μm (sectors)
