Figure 5
NF-κB is essential for XIAP-mediated suppression of ROS and inhibition enhances ADCC. (a) Percentage of wtXIAP cells with high hydrogen peroxide-derived radicals in cells treated as indicated. Bars represent mean±S.E.M. relative to untreated cells, n=2–3. (b) Clonogenic growth assay in cells treated as indicated. Bars represent mean±S.E.M. colonies formed/cells plated as a percentage of the untreated sample, n=2–3. (c) Western immunoblotting for phospho-p65 (p-NF-κB), total p65 (NF-κB) and GAPDH as loading control in SUM149 and wtXIAP cells treated with vehicle or 1 μM NRAGE peptide. (d) Percent cell lysis of wtXIAP cells incubated with cetuximab alone or in ADCC conditions in the presence or absence of NRAGE peptide. Bars represent mean±S.E.M. calculated percent lysis, n=2–3. *P<0.05, **P<0.005, #P<0.001
