Figure 1
Induced Autophagy in SH-SY5Y and HSG cells. (a) HSG and SHSY-5Y cells were treated for 24 h with 1 mM DFO, 1 mM DMOG, and in a serum-free media. Protein was isolated and western blots represent the protein expression of different autophagy marker beclin-1, LC3A, p62, and loading control actin. (b) Corresponding densitometric reading of the autophagy marker protein is shown as a bar diagram. Each bar gives the mean±S.E.M. of four separate experiments. *P<0.05, **P<0.01, and ***P<0.001. Confocal image of HSG and SH-SY5Y cells, respectively, transfected with fluorescent-tagged LC3 and treated for 24 h with 200μM DFO or 200μM DMOG. (c and d) Western blot images showing the expression of autophagy marker LC3A in primary salivary gland cells isolated from normoxia- and hypoxia-induced mice models. (e) Bar diagram representing the densitometric reading of the LC3A in the above-mentioned western blots. Each bar gives the mean±S.E.M. of four separate experiments
