Figure 4

TRPC channel inhibitors attenuate the increase intracellular calcium in autophagy induces cells. (a) Representative traces showing the transient increase in [Ca²⁺]_i after the addition of 1 mM calcium in the presence of 50 μM 2APB to SHSY-5Y cells pretreated with 1 mM DMOG. Bar diagram shows the [Ca²⁺]_i in nM concentration of the above-mentioned experiment. Each bar gives the mean±S.E.M. of 50 separate experiments. e **P<0.01 and ***P<0.001. (b) Representative traces showing the transient increase in [Ca²⁺]_i after the addition of 1 mM calcium in the presence of 10 μM SKF to SHSY-5Y cells pretreated with 1 mM DMOG. Bar diagram shows the [Ca²⁺]_i in nM concentration of the above-mentioned experiment. Each bar gives the mean±S.E.M. of 50 separate experiments. **P<0.01 and ***P<0.001. (c) Bar diagram showing the cell viability assay (MTT assay) in the SHSY-5Y cells, pretreated with 1 mM DMOG in the presence of 10 μM SKF. Each bar gives the mean±S.E.M. of four separate experiments. **P<0.01 and ***P<0.001. (d) Western blot images showing the expression of LC3A, caspase 3 in SHSY-5Y cells pretreated with 1 mM DMOG and 10 μM SKF. Confocal image of HSG (e) and SH-SY5Y (f) cells transfected with GFP-LC3 and treated with 200 μM DFO or 200 μM DMOG and 10 μM SKF