Figure 5
Increases in TRPC1 expression and currents in autophagy-induced HSG and SHSY-5Y cells. (a and b) Application of 1 μM Tg in bath solution induced inward currents at −80 mV in control, 1 mM DMOG-, and 1 mM DFO-treated cells. (c and d) Respectively I–V curves under these conditions are shown in c and quantitation (8–10 recordings) of current intensity at −80 mV is shown in d, *P=<0.05. (e) I–V curves of currents induced by the application of 1 μM Tg in standard external Ringer’s solution and Na-based DVF external solutions. (f) Bath application of 1 μM Tg in bath solution induced in salivary gland cells and relative I–V curves. (g) Average (8–10 recordings) current intensity at −80 mV under these conditions is shown, *P=<0.05. (h) Represents western blot images showing the expression of SOCE components, STIM1, Orai1, and TRPC1 in HSG and SHSY-5Y cells pretreated with 1 mM DMOG and 1 mM DFO for 24 h. Corresponding densitometric reading of the protein is shown as a bar diagram (i). Each bar gives the mean±S.E.M. of four separate experiments. *P<0.05, **P<0.01, and ***P<0.001. (j) Western blot images showing the expression of TRPC1 in primary salivary gland cells isolated from control and hypoxia-induced mice models. Bar diagram representing the densitometric reading of the TRPC1 in the above-mentioned western blots. Each bar gives the mean±S.E.M. of four separate experiments. (k) Western blot images showing the relative surface expression of TRPC1 obtained from cell surface biotinylation. Bar diagram shows the normalized expression of TRPC1 to the expression of cell surface transferrin receptor (TfR) protein. Each bar gives the mean±S.E.M. of three separate experiments
