Figure 7
Pretreatment with autophagy inhibitor 3-methyladenine (3-MA) attenuated the intracellular calcium influx and induces apoptosis. (a) Western blot images showing the expression of SOCE components STIM1, TRPC1, and Orai1, autophagy marker beclin-1 and loading control actin in HSG and SHSY-5Y cells pretreated with 1 mM DMOG or in serum-free media in the presence of 1 mM autophagy marker 3-MA for 24 h. (b) Representative traces showing the transient increase in [Ca2+]i after the addition of 1 mM calcium in the presence of 1 mM 3-MA to SHSY-5Y cells pretreated with 1 mM DMOG or in serum-free media. (c) Bar diagram shows the [Ca2+]i in nM concentration of the above-mentioned experiment. Each bar gives the mean±S.E.M. of 50 separate experiments. *P<0.05, **P<0.01. (d) Bar diagram showing the cell viability assay (MTT assay) in the SH-SY5Y cells, pretreated with 1 mM DMOG in the presence of 1 mM. Each bar gives the mean±S.E.M. of four separate experiments. ***P<0.001. (e) Application of 1 μM Tg in bath solution induced inward currents at −80 mV in control, cells treated in serum-free media and autophagy inhibitor 3-MA-treated cells. (f) Average (8–10) recordings current intensity at −80 mV are shown, *P<0.05
