Figure 1 | Cell Death & Disease

Figure 1

From: Hyperhomocysteinemia causes ER stress and impaired autophagy that is reversed by Vitamin B supplementation

Figure 1

Hcy inhibited autophagy in primary mouse astrocytes. Primary astrocytes were isolated and cultured as described in materials and methods. (a) western blot analysis of primary mouse astrocytes treated with different Hcy concentrations as mentioned in the figure panel for 48 h. (b and c) Quantitative analysis of MAP1LC3B-II and SQSTM1, respectively, was done and plotted as bar graphs. (d) Western blot analysis of primary mouse astrocytes treated with 2.0 mM Hcy for different time points as mentioned in the figure panel. (e and f) Quantitative analysis of MAP1LC3B-II and SQSTM1, respectively was done and plotted as bar graphs. (g) Autophagic flux analysis was done by using Bafilomycine A1 (50 nM for 6 h) along with or without Hcy (2.0 mM for 48 h). (h) Quantitative analysis of MAP1LC3B-II was done and plotted as bar graphs. The data are shown as Mean±S.D. and statistical difference *P<0.05 was considered as significant

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