Figure 2 | Cell Death & Disease

Figure 2

From: Hyperhomocysteinemia causes ER stress and impaired autophagy that is reversed by Vitamin B supplementation

Figure 2

Hcy increased MTOR signaling and inhibited lysosomal pathway in primary mouse astrocytes. Primary astrocytes were isolated and cultured as described in materials and methods. (a) Western blot analysis of primary mouse astrocytes treated with different Hcy concentrations as mentioned in the figure panel for 48 h. (b) Quantitative analysis of phospho-MTOR, RPS6KB1 and EIF4EBP1 proteins was done and plotted as bar graphs. (c) Western blot analysis of primary mouse astrocytes treated with 2.0 mM Hcy for different time points as mentioned in the figure panel. (d) Quantitative analysis of phospho-MTOR, RPS6KB1 and EIF4EBP1 proteins was done and plotted as bar graphs. (e) Primary mouse astrocytes treated with Hcy (2.0 mM for 48 h), RT-qPCR analysis of key lysosomal and autophagic genes was performed and plotted as bar graph. (f) Western blot analysis of primary mouse astrocytes treated with Hcy (2.0 mM for 48 h). (g) Quantitative analysis of western blots in f was done and plotted as bar graphs. The data are shown as Mean±S.D. and statistical difference *P<0.05 was considered as significant

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