Figure 3
Hcy increased ER stress in primary mouse astrocytes. Primary astrocytes were isolated and cultured as described in materials and methods. (a) Western blot analysis of primary mouse astrocytes treated with different Hcy concentrations as mentioned in the figure panel for 48 h. (b) Quantitative analysis of ER stress marker proteins was done and plotted as bar graphs. (c) Western blot analysis of primary mouse astrocytes treated with 2.0 mM Hcy for different time points as mentioned in the figure panel. (d) Quantitative analysis of ER stress marker proteins was done and plotted as bar graphs. (e) Primary mouse astrocytes treated with Hcy (2.0 mM for 48 h), RT-qPCR analysis of key ER stress genes was performed and plotted as bar graph. The data are shown as Mean±S.D. and statistical difference *P<0.05 was considered as significant
