Figure 6 | Cell Death & Disease

Figure 6

From: Hyperhomocysteinemia causes ER stress and impaired autophagy that is reversed by Vitamin B supplementation

Figure 6

Vitamin supplementation during diet-induced HHcy reversed autophagic block, MTOR signaling activation lysosomal dysfunction and alleviated ER stress in mouse brain. (a, d, f, and h) Western blot analysis of brain tissue isolated from mouse fed on control diet, diet rich in Methionine and deficient in vitamin supplementation (M+B−) caused HHcy; and diet rich in methionine as well as 3 × supplemented with vitamin (M+B+). (b and c) Quantitative analysis of MAP1LC3B-II and SQSTM1, respectively, was done and plotted as bar graphs. (e, g, and i) Quantitative analysis of western blots in d (for MTOR signaling), f (for lysosomal pathway), and i (for ER Stress) are plotted as bar graphs. The data are shown as Mean±S.E.M. and statistical difference *P<0.05 was considered as significant. (j) RT-qPCR analysis of mouse brain tissues to analyze key genes of lysosomal pathway and ER stress. The data are shown as Mean±S.E.M. and statistical difference *P<0.05 was considered as significant

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