Figure 7

MTOR inhibitor rapamycin treatment reversed Hcy-induced ER stress and inhibition in autophagy. (a, c and e) Western blot analysis of primary human astrocytes treated with Hcy (2.0 mM for 48 h) alone or along with rapamycin (50 μM) for autophagy, MTOR signaling and ER stress, respectively. (b, d and f) Quantitative analysis of western blots was done and plotted as bar graphs. The data are shown as Mean±S.D. and statistical difference *P<0.05 was considered as significant