Figure 5
From: Dual targeting of glutaminase 1 and thymidylate synthase elicits death synergistically in NSCLC
Cell death was significantly induced by dual treatment with BPTES and 5-FU through cell cycle arrest. To test the effect of combined BPTES and 5-FU treatment, NSCLC cells were treated with 10 μM of BPTES, 10 μM of 5-FU, or both 10 μM of BPTES and 10 μM of 5-FU for 48 h. (a) Cell cycle was measured by FACS analysis using PI staining after GLS1 inhibition with 10 μM of BPTES and/or 10 μM of 5-FU for 12 h in A549 cells. (b) Cell cycle was measured by FACS analysis using PI staining after GLS1 knockdown with 10 μM of BPTES and/or 10 μM of 5-FU for 12 h in EKVX cells. (c) Cell death was measured with FACS analysis using PI and annexin V in A549 after drug treatment for 48 h. (d) Cell death was measured with FACS analysis using PI and annexin V in EKVX after drug treatment for 48 h. (e) Cell death was measured with TUNEL staining in A549 after drug treatment for 48 h. The scale bar, 50 μm. (f) Cell death was measured by the annexin V assay after cells were treated with 10 μM of BPTES, 10 μM of 5-FU, or combined for 12, 24 and 48 h in A549 and IMR90. P-values were determined using two-tailed Student’s t-tests (ns, not significant; *0.01<P<0.05; **0.001<P<0.01; ***P<0.001)
