Figure 2 | Cell Death & Disease

Figure 2

From: Platinum pyrithione induces apoptosis in chronic myeloid leukemia cells resistant to imatinib via DUB inhibition-dependent caspase activation and Bcr-Abl downregulation

Figure 2

PtPT treatment downregulates Bcr-Abl and its downstream signaling proteins. (a and b) PtPT decreases the protein levels of Bcr-Abl and its downstream targets in a dose- and time-dependent manner. CML cells were treated with PtPT as indicated, then collected for western blot analyses for the indicated proteins. (c) PtPT decreases mRNA expression of Bcr-Abl. KBM5 and KBM5R cells were exposed to 1.0 μM PtPT for 9 or 12 h. The Bcr-Abl mRNA level was determined using RT2-PCR and its expression level relative to the control was calculated. Mean±S.D. (n=3). **P<0.01, ***P<0.001, versus control group. (d) PtPT inhibits cellular activity of RNA pol II. KBM5 and KBM5R cells were treated with PtPT (1 μM) for the indicated duration, the phosphorylated (p-Rpb) and total protein levels of RNA pol II (Rpb) were analyzed with western blot

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