Figure 6

Syndecan-2 overexpression altered angiogenesis. Flow cytometric analysis of CD31 and Endomucin-stained marrow cells. (a) Cells that did not express the markers of hematopoietic lineages (blue gate, Lin⁻) have been selected for the analysis of CD31 and Endomucin expression. (B and F) Representative dot plots of CD31 and Endomucin staining in WT (C and D) or transgenic mice (E and F) at 6 weeks (C and E) or 12 weeks (D and F) of age. P5 gate was placed arbitrarily to select cells with the higher levels of CD31 and Endomucin. (b) Quantification of the % of Lin⁻ cells with CD31 staining. (c) Quantification of the % of Lin⁻ cells with Endomucin staining. (d) Quantification of the % of Lin⁻ cells that expressed high levels of CD31 and Endomucin (P5 gate). Results are the mean %±S.E.M. (seven WT and three ColI-Synd2 for 6-week-old mice; four WT and four ColI-Synd2 for 12-week-old mice). * indicates significant difference between WT and ColI-Synd2 (P<0.05). (e) Representative photos of analysis of Endomucin-expressing vessels within the bone marrow of 6-week-old (A and B) or 4-month-old (C and D) mice. Sections of paraffin-embedded vertebra from WT (A and C) or transgenic (B and D) mice were stained with an anti-endomucin antibody and green fluorescence-conjugated secondary antibody. DAPI was used to stain the nuclei. Control sections were incubated with rat Ig (E). Arrows indicate the vessels with Endomucin⁺ cells