Figure 6
STAT3 phosphorylation is inhibited by Lyn activation in DLBCL. (a) OCI-LY8 and NU-DUL-1 cells were treated with DCZ3301 (8 and 16 μM) for 48 h and assessed the protein levels of Lyn, phospho-Lyn, Syk, phospho-Syk and Actin by performing western blotting analysis. (b) The protein levels of Lyn, phospho-Lyn, STAT3, phospho-STAT3 and Actin in OCI-LY8 cells treated with DCZ3301 (16 μM) for 12, 24, 36 and 48 h were examined by western blotting analysis. (c) OCI-LY8 and NU-DUL-1 cells were transfected with a plasmid that overexpresses oncogene Lyn and an empty vector, respectively. The protein levels of Lyn and Actin were analyzed by western blotting. (d) The transfected OCI-LY8 and NU-DUL-1 cells were treated with DCZ3301 (0 and 16 μM) for 24 h and the protein levels of Lyn, phospho-STAT3 and Actin were examined by western blotting analysis. (e) The signal transduction pathway involving in STAT3 activation that DCZ3301 induced in cell apoptosis of DLBCL cells
