Figure 3

CXCL1, CXCL2 and CXCR2 interaction mediates the recruitment of MDSCs in injured kidney. (a) The expression of chemokine CXCL1, CXCL2 mRNA in kidney were upregulated on POD 1 and POD 2, but the expression of CXCL3 and CXCL7 mRNA were not (the expression was normalized to GAPDH and relative to control). (b) The percentage of CD11b+CXCR2+ cells in the kidney on POD 1 and POD 2 were increased in the rapamycin-treated group compared with sham group and the IR group. Cells in the kidney were gated first in FSC/SSC, and then CD11b+CXCR2+ cells were detected by flow cytometry. (data were shown as mean±S.D.; n=5 mice per group per time point; POD, postoperative day)